By comparison, Dynamic Light Scattering (DLS) takes advantage of the combined motion of a great group of randomly oriented particles spread in some medium.ĭLS depends on the fact that easily diffusing particles, travelling randomly because of Brownian motion, will result in quick variations in scattered laser light. This technique is employed to achieve parameters like M w, R g and A 2. Static Light Scattering (SLS) needs very precise photon counting, resulting in the magnitude of the scattered light becoming the most essential parameter. Static Light ScatteringĬommercially available light scattering instruments usually take advantage of one of two fundamental values to obtain data from this scattered light. Provided Δn is nonzero, light scattering should take place.

The strength of light scattered is relative to size, molecular weight and the variation in refractive index (Δn) across the scattering center (n sample) and solvent (n solvent). Inhomogeneities cause scattered light in a uniform continuum, there would not be any deflection of the path of laser light as it moves through a medium. Light scattering is something that can be seen when light, typically monochromatic laser light, is scattered by objects oriented at random in solution. Image Credit: Brookhaven Instrument Corporationĭynamic Light Scattering (DLS) is a measurement method that offers a quick and easy technique for the sizing of submicron and nanoparticle. Sponsored by Brookhaven Instruments Corporation Dec 24 2020

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